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topB [2018-02-12 16:01:58]
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topB [2018-02-12 16:01:58]

DNA topoisomerase III
locus
BSU04260
pI
9.88
mw
81.29 kDa
protein length
727 aa Sequence Blast
gene length
2181 bp Sequence Blast
function
replication
product
DNA topoisomerase III
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

    • Gene

    Coordinates
    476,558 → 478,741

    The protein

    Catalyzed reaction/ biological activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)

    • Protein family

  • topo IA family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|TopA]

    • [SW|Domains]

  • N-terminal [SW|TOPRIM domain] (aa 3-136) [Pubmed|9722641]

    • Structure

  • [PDB|1D6M] (from ''E. coli'', 33% identity) [Pubmed|10574789]

    • Expression and Regulation

    Operons

    genes
    [gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

    description

  • [pubmed|22383849]
    • view in new tab

    genes
    [gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

    description

  • [pubmed|22383849]

    • sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|10913073], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
  • BKE04260 (''topB''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • GP1864 (''topB''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
  • GP1896 (''topB''::''cat'') (available in [SW|Jörg Stülke]'s lab)
  • JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
  • BKE04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
  • BKK04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT

    • References

  • 17114254,16600296,9722641,10574789