topB [2018-02-12 16:01:58]

DNA topoisomerase III

Locus: BSU04260

Isoelectric point: 9.88

Molecular weight: 81.29 kDa

Protein length: 727 aa

Gene length: 2181 bp

Function: replication

Product: DNA topoisomerase III

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → Genetics → DNA condensation/ segregation

Gene

Coordinates: 476,558 → 478,741

The protein

Catalyzed reaction/ biological activity

• ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)

Protein family

• topo IA family (according to Swiss-Prot)

Paralogous protein(s)

• TopA

Domains

• N-terminal TOPRIM domain (aa 3-136) PubMed

Structure

• 1D6M (from E. coli, 33% identity) PubMed

Expression and Regulation

Operons

• Genes: topB

  Description

  • PubMed

• Genes: lrpC-topB

  Description

  • PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• MGNA-C111 (topB::erm), available at the NBRP B. subtilis, Japan
• BKE04260 (topB::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
• GP1864 (topB::ermC) (available in Jörg Stülke's lab)
• GP1896 (topB::cat) (available in Jörg Stülke's lab)
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04260 (ΔtopB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
• BKK04260 (ΔtopB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT

References

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